This is a knockout-validated antibody summary, based on the publications "Squalene accumulation in cholesterol auxotrophic lymphomas prevents oxidative cell death", as cited below [1], and "Genome-scale CRISPR screening for modifiers of cellular LDL uptake" for western blot knockout validation (figure 4b) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Rabbit monoclonal IgG

Company: Abcam

Antibody: LDL Receptor

Catalog number: ab52818

Summary: Rabbit monoclonal IgG against a synthetic peptide within Human LDL Receptor (aa 800 to the C-terminus). Reacts with mouse and human. Suitable for western blot and immunohistochemistry (paraffin).

Western blot

Control and LDLR-KO Karpas299 cells. Cells were lysed in buffer (10 mM Tris-Cl pH 7.5, 150 NaCl, 1 mM EDTA, 1% Triton X-100, 2% SDS, CHAPS 0.1%) supplemented with protease inhibitors and PhosSTOP (Roche).

HRP-conjugated anti-rabbit antibody (Santa Cruz).

Immunoblots for LDLR and SQLE in control and SQLE cDNA expressing Karpas299 cells infected with sgAAVS1 or sgLDLR1 virus in the presence or absence of Shield-1 (250 nM). Please see Ext. Figure 4e in the article [1].