This is a knockout-validated antibody summary, based on the publication "Autophagy deficiency exacerbates acute lung injury induced by copper oxide nanoparticles", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Wild type and lc3b-/- mice were intratracheally instilled of vehicle or 5 mg/kg CuONPs, respectively, then the lung tissues and BALFs of indicated groups were collected 3 days after treatment. The protein expression levels of LC3B-I/II in the mice lung tissues were determined by western blot analysis. β-actin served as internal control. From [1].

Rabbit monoclonal IgG

Company: Abcam

Antibody: LC3B

Catalog number: ab192890

Summary: Rabbit monoclonal antibody against a synthetic peptide of LC3B. Reacts with mouse, rat and human. Suitable for western blot, immunoprecipitation, immunocytochemistry/immunofluorescence, and immunohistochemistry (paraffin).

Western blot

WT and lc3b-/- mice lung tissues and bronchoalveolar lavage fluids (BALFs).

5% nonfat milk in TBST.

1:3,000 dilution overnight at 4 °C.

HRP-conjugated secondary antibodies.

ChemiDoc Touch Imaging system (1708370, Bio-Rad).