This is a knockout-validated antibody summary, based on the publication "Genetic Disruption of the Multifunctional CD98/LAT1 Complex Demonstrates the Key Role of Essential Amino Acid Transport in the Control of mTORC1 and Tumor Growth", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Rabbit polyclonal

Company: TransGenic Inc.

Antibody: LAT1

Catalog number: KE026

Summary: Rabbit polyclonal antibody raised against a synthetic peptide corresponding to the C-terminal region of human LAT.

The antibody was antigen affinity purified.

Western blot

Immunofluorescence

Western blot: Lysates of wild-type and LAT1 -/- LS174T human colon adenocarcinoma cells.

Immunofluorescence: Wild-type and LAT1 -/- LS174T cells.

Fixed with 3% paraformaldehyde and permeabilised with 0.2% Triton X-100 in PBS.

Western blot: 5% milk powder in TBS.

Immunofluorescence: Not stated.

Western blot: 1:1000 dilution.

Immunofluorescence

Western blot: HRP-conjugated anti-rabbit antibody (Promega).

Immunofluorescence: 1:1000 dilution of FluoProbe594-conjugated anti-rabbit antibody (FP-SD 5110-T, Interchim).

Western blot: ECL (Merck Millipore) in conjunction with LI-COR Odyssey Imaging System.

Immunofluorescence: Leica SP5 confocal microscope with LS AF and Image J software.

See figure 1 from the article [1].

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.