This is a knockout-validated antibody summary, based on the publication "Lysosomal putative RNA transporter SIDT2 mediates direct uptake of RNA by lysosomes", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Immunoblot of LAMP2 levels in LAMP2-deficient HeLa cells and parental HeLa cells (control HeLa). Beta-actin was used as loading control. From [1].

Rat monoclonal IgG1

Company: Santa Cruz Biotechnology

Antibody: LAMP2

Catalog number: sc-19991

Summary: Rat monoclonal IgG1 against epitope mapping to immunoadsorbent-purified mouse macrophage glycoprotein fraction. Recommended for detection of LAMP-2 of mouse, rat, human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and flow cytometry.

Western blot

HeLa and LAMP2-deficient HeLa cells. Cells were lysed or homogenized in lysis buffer containing 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5 mM EDTA, 1% Triton X-100 (Sigma-Aldrich, T8787) and protease inhibitors (Complete, EDTA-free; Roche Diagnostics, 11873580001) at 4°C.

Overnight at 4°C

Horseradish peroxidase (HRP)-conjugated anti-rat IgG antibodies (Jackson ImmunoResearch, 112-035-003).

Using SuperSignal West Dura extended duration substrate (Thermo Scientific, 34075) and detected with a chemiluminescence imaging system (ImageQuant LAS 4000; Fujifilm).

The same clone (M3/84) is sold as BioLegend 108501, 108502, 108504, 108505, 108506, 108508, 108510, 108511, 108512; US Biological L9190-12E, L9190-12J; Santa Cruz Biotechnology sc-19991; LifeSpan Biosciences LS-C8583, LS-C8584, LS-C62619, LS-C62620, LS-C62621, LS-C105780, LS-C107115, LS-C107138, LS-C122073, LS-C130576; Abnova MAB10954; Invitrogen MA5-17861, MA5-17862, MA5-17863, MA5-17864, MA5-17865, MA5-17866, MA5-17867, MA5-17868, A18535; Antibodies-online ABIN120162; BD Biosciences 562061, 553324, 553323, 553322, 550292; EMD Millipore MABC24.