This is a knockout-validated antibody summary, based on the publication "Molecular, Cellular and Functional Changes in the Retinas of Young Adult Mice Lacking the Voltage-Gated K+ Channel Subunits Kv8.2 and K2.1", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Retinal localization and expression of the voltage-gated K+ channel protein subunits Kv8.2 and Kv2.1 in wild type (WT), Kv8.2 knockout (KO) and Kv2.1 KO. Representative confocal images of the inner segment (IS) area, outer nuclear layer (ONL), inner nuclear layer (INL) and ganglion cell layer (GCL) showing expression of Kv8.2 and Kv2.1 mouse subunits in wild type (A), Kv8.2 KO (B) and Kv2.1 KO (C) retinas at 20× (top panels) and 40× (bottom panels) magnification. Scale bars = 20 μM (top panels) and 50 μM (bottom panels). From [1].

Mouse monoclonal IgG1

Company: NeuroMab

Antibody: Kv2.1 potassium channel subunit

Catalog number: 75-014

Summary: Mouse monoclonal antibody against a synthetic peptide of rat Kv2.1 (amino acids 837-853). Reacts with mouse, human and rat. Suitable for western blot, immunohistochemistry and immunoprecipitation.

Immunohistochemistry

WT and Kv2.1-KO mouse retinas.

10% normal goat serum, 0.5% Triton X-100, (Sigma-Aldrich, St. Louis, MO, USA) 1% BSA (Sigma-Aldrich, St. Louis, MO, USA) in 1X PBS for 1 h at room temperature.

1:1,000 dilution in blocking solution overnight at room temperature.

1:500 dilution goat AlexaFluor-488 (Abcam, Cambridge, MA, USA), or -568 (Abcam, Cambridge, MA, USA) diluted in block solution for 2 h at room temperature.

Nikon A1Si confocal microscope.

The same clone (K89/34) is sold as OriGene 75-014; Abcam ab192761; Neuromab 73-014, 75-014.