This is a knockout-validated antibody summary, based on the publication "Bax deficiency extends the survival of Ku70 knockout mice that develop lung and heart diseases", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Immunoblot of Ku70 in WT, ku70−/−, ku70−/−bax+/− and ku70−/−bax−/− MEFs showing the protein expression levels of Ku70, Bax, and actin (loading control). From [1].

Company: NeoMarkers

Antibody: Ku70

Catalog number: N3H10

Summary: Mouse monoclonal IgG2b against human placental extract designated as PSE1-PL. Reacts with human , mouse , primate, and clawed frog. Suitable for western blot, immunocytochemistry, immunofluorescence, immunohistochemistry (paraffin), immunoprecipitation, and flow cytometry. Reacts with mouse and hamster in western blot applications only.

Western blot

WT, WT, ku70−/−, ku70−/−bax+/− and ku70−/−bax−/− MEFs. Cells were RIPA lysis buffer (150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, and 0.1% SDS in PBS) containing a protease inhibitor cocktail (Thermo Scientific, Waltham, MA, USA; no. 87786) and PMSF for 20 min at 4°C.

5% BSA, 3% milk, and 2% normal serum of the host species from which the secondary antibody was derived for 1 h at room temperature.

1:500 for 1 h at room temperature or overnight at 4°C

Horseradish peroxidase-conjugated goat anti-mouse (Life Technologies) for 1 h at room temperature.

ECL detection system (GE Healthcare, Pittsburgh, PA, USA; RPN2132).

The same clone (N3H10) is sold as Novus Biologicals NB100-1915; Santa Cruz Biotechnology sc-56129; LifeSpan Biosciences LS-C83596, LS-C88086, LS-B8606, LS-C170895; Abnova MAB1979; Invitrogen MA5-13110.