This is a knockout-validated antibody summary, based on the publication "Kinesin-1 controls mast cell degranulation and anaphylaxis through PI3K-dependent recruitment to the granular Slp3/Rab27b complex", as cited below [1]. This clone was also validated for Western blot in the article "A small-molecule activator of kinesin-1 drives remodeling of the microtubule network" (see figure 3c) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Immunocytochemistry of Kif5b and tubulin in WT (F) and cKOKif5b (G) BMMCs plated on fibronectin-coated glass coverslips. NS, not stimulated. Bars, 2 µm. From [1]. Confirmed additionally by the author.

Mouse IgG1

Company: BioLegend / DSHB

Antibody: Kif5b

Catalog number: SUK 4

Summary: This product is discontinued from BioLegend. The same clone is available from DSHB.

Note: The clone name is SUK 4.

Immunocytochemistry

WT and Kif5b-cKO bone marrow–derived mast cells (BMMCs).

ICC: in permeabilization buffer (PBS with 1 mg/ml bovine serum albumin and 0.05% wt/vol saponin; Sigma-Aldrich) for 1 h.

ICC: fluorescent-conjugated secondary antibody in permeabilization buffer for 1 hour

ICC: Confocal microscopy was performed with an LSM 700 system (ZEISS) and 63× NA 1.4 objective.