This is a knockout-validated antibody summary, based on the publications "The cell proliferation antigen Ki-67 organises heterochromatin", as cited below [1], and "Ki-67 regulates global gene expression and promotes sequential stages of carcinogenesis" for western blot knockout validation (figure s4c,s5c) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Immunohistochemical staining of Ki67 in sections of small intestine from wild-type, Ki67 +/- and Ki67 -/- mice. From [1].

Rabbit monoclonal

Company: Abcam

Antibody: Ki67

Catalog number: ab16667 Clone Sp6

Summary: Rabbit polyclonal raised against a (proprietory) synthetic peptide sequence within residues 2300 – 2400 of human Ki67. Reacts with mouse, rat, human and marmoset Ki67.

Supplier recommended for IHC, ICC and WB.

Immunohistochemistry

Freshly dissected small intestine from wild-type and Ki67 -/- mice.

Formalin fixed and paraffin embedded.

TBS pH 7.4 containing 5% dried milk powder and -.5% Triton X-100.

Blocked for 1 hour at room temperature.

Overnight in blocking buffer (1:300).

Fluorescently labelled secondary antibody in PBS containing 0.1% Triton X-100.

DAB with hemotoxylin counter staining.

The same clone (SP6) is sold as Novus Biologicals NB600-1252; OriGene DRM004; Abcam ab16667, ab21700, ab197547, ab231172, ab281847, ab281928, ab282173; Rockland Immunochemicals 900-C01-B38; Invitrogen MA5-14520; GeneTex GTX16667; Biocare Medical CRM 325; Cell Marque 275R-14, 275R-18; MilliporeSigma SAB5500134; Arigo ARG53222.