Keratin-8 antibody | knockout validation | NeoMarkers (Thermo Fisher) MA5-14428

DOI

//dx.doi.org/10.13070/ko.en.6.1869

Date

2016-11-25

This is a knockout-validated antibody summary, based on the publication "Keratins modulate c-Flip/extracellular signal-regulated kinase 1 and 2 antiapoptotic signaling in simple epithelial cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Mouse monoclonal

Company: NeoMarkers (Thermo Fisher Scientific)

Antibody: Keratin-8

Catalog number: MA5-14428 (Clone TS1)

Summary: Mouse monoclonal antibody raised against a human carcinoma keratin preparation. Supplier recommended for flow cytometry, ICC, IF, IHC and IP. Reacts with human keratin-8.

Validation Method

Western blot

Sample

Lysates of tissues from wild-type and keratin-8 null mice.

Primary incubation

Anti-keratin-8 antibody.

Secondary incubation

HRP-conjugated secondary antibody.

Detection

Super Signal West Pico ECL reagent (Pierce).

Figure

See Figure 3 in [1].

Clone note

The same clone (TS1) is sold as US Biological 168753, 168753-AF; LifeSpan Biosciences LS-C88563, LS-C88564, LS-C88565, LS-C189543, LS-C189544, LS-C189545, LS-C357847; Abnova MAB12135; Invitrogen MA5-14425, MA5-14428.

References

Gilbert S, Loranger A, Marceau N. Keratins modulate c-Flip/extracellular signal-regulated kinase 1 and 2 antiapoptotic signaling in simple epithelial cells. Mol Cell Biol. 2004;24:7072-81 pubmed