This is a knockout-validated antibody summary, based on the publication "Keratin 17 Is Induced in Oral Cancer and Facilitates Tumor Growth", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot analysis of KRT17 in wild type and KRT17-KO HSC3 cells. GAPDH was used as loading control. From [1].

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: KRT17

Catalog number: 4543

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to amino acids near the carboxy terminus of human keratin 17. Reacts with human, mouse, and rat. Suitable for western blot, immunocytochemistry and flow cytometry. It is predicted to react based on 100% sequence homology with monkey and dog.

Western blot

Wild type and KRT17-KO HSC3 cells. Protein extraction from cells was carried out using RIPA or NP40 lysis buffer.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.