This is a knockout-validated antibody summary, based on the publication "Krüppel-Like Factor 4 (KLF4) Is Not Required for Retinal Cell Differentiation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot of KLF4 protein expression in the retinae of KLF4 cKO and control mice (WT). GAPDH is used as loading control. From [1].

Antibody: KLF4

Catalog number: ab72543

Summary: Rabbit polyclonal raised against a synthetic peptide corresponding to a region between residues 1-50 of human KLF4. Reacts with protein from mouse, rat, cow, human, and monkey; and has been used in western blot, immunohistochemistry-paraffin and flow cytometry.

Company: Abcam

Western blot

Retinae of KLF4 cKO and control mice, lysed in lysis buffer (Cell Signaling Technology) containing 0.5 mm phenylmethylsulfonyl fluoride (Sigma-Aldrich)

5% nonfat milk powder and 0.1% Tween-20, pH 7.6, for 1 h

overnight incubation at 4C, dilution 1:50

horseradish peroxidase (HRP)-conjugated anti-goat or rabbit IgG (1:2000; Santa Cruz Biotechnology)

chemiluminescence substrate for HRP (Thermo Fisher Scientific). The images of Western blots were captured by GE ImageQuant.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.