KIF5B antibody | knockout validation | Abcam ab167429
DOI
//dx.doi.org/10.13070/ko.en.6.1780
Date
2016-11-07

This is a knockout-validated antibody summary, based on the publication "Dynamic tubulation of mitochondria drives mitochondrial network formation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

KIF5B antibody | knockout validation | Abcam ab167429 figure 1
Figure 1. Western blots of WT and KIF5B NRK cell lysates probed with anti-KIF5B antibody (Abcam, Cat no. ab167429). Actin served as a loading control. From [1].
Antibody information

Rabbit monoclonal

Company: Abcam

Antibody: KIF5B

Catalog number: Ab167429 Clone EPR10276(B)

Summary: Raised against a peptide within residues 1-100 of human KIF5B. It detects human, mouse and rat KIF5B. Supplier recommended for Western blotting and IP, IHC and ICC. Supplier states the antibody is unsuitable for flow cytometry.

Validation Method

Western blotting

Sample

Wild-type and KIF5B -/- NRK cell lysates.

Primary incubation

Anti-KIF5B.

Disclaimer

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.

References
  1. Wang C, Du W, Su Q, Zhu M, Feng P, Li Y, et al. Dynamic tubulation of mitochondria drives mitochondrial network formation. Cell Res. 2015;25:1108-20 pubmed publisher