KIF3A antibody | knockout validation | Cell Signaling 8507

This is a knockout-validated antibody summary, based on the publication "Activation of Cilia-Independent Hedgehog/GLI1 Signaling as a Novel Concept for Neuroblastoma Therapy", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

KIF3A antibody | knockout validation | Cell Signaling 8507 figure 1
Figure 1. Immunoblot depicting the protein levels of GLI1 and KIF3A in Kif3a wt/KO NIH3T3 cells. Treatment was for 48 h with 100 nM SAG or 20 µM ISX. Shown is one of three independent experiments. Tubulin was used for normalization. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: KIF3A

Catalog number: 8507

Summary: Rabbit monoclonal antibody against a synthetic peptide corresponding to residues near the carboxy terminus of human KIF3A protein. Reacts with human, mouse, rat, and monkey. Suitable for western blot and immunoprecipitation.

Validation Method

Western blot

Sample

WT and KIF3A KO NIH3T3 cells.

Secondary incubation

Horseradish peroxidase (HRP)-coupled secondary antibody (CST, #7076).

Detection

Pierce ECL Western Blotting Substrate (Thermo Scientific) according to the manufacturer’s protocol.

References
  1. Koeniger A, Brichkina A, Nee I, Dempwolff L, Hupfer A, Galperin I, et al. Activation of Cilia-Independent Hedgehog/GLI1 Signaling as a Novel Concept for Neuroblastoma Therapy. Cancers (Basel). 2021;13: pubmed publisher