This is a knockout-validated antibody summary, based on the publication "Obacunone protects retinal pigment epithelium cells from ultra-violet radiation-induced oxidative injury", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Stable ARPE-19 cells with the CRISPR/Cas9-Keap1-KO construct (“ko-Keap1”) or the empty vector (“Cas9-C”) were treated with or without obacunone (25 μM), cells were further cultured for 6h, expression of listed genes was shown. From [1].

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: KEAP1

Catalog number: 8047

Summary: Rabbit monoclonal antibody against a synthetic peptide corresponding to residues near the carboxy terminus of human KEAP1 protein. Reacts with human, mouse, and rat. It is predicted to react based on 100% sequence homology with monkey, bovine, and pig. Suitable for western blot and immunofluorescence (immunocytochemistry).

Western blot

Controla and Keap1 KO APRE-19 cells.