This is a knockout-validated antibody summary, based on the publication "An easy and efficient inducible CRISPR/Cas9 platform with improved specificity for multiple gene targeting", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. HeLa/iCas9-c1 cells were transduced with LentiGuide or Lenti-entry-puro carrying the indicated sgRNA(s). Cells were then treated with or without 1 μg/ml DOX for 48 h and harvested for western blot analyses. Vinculin was used as loading control. From [1].

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: KDM5A

Catalog number: 3876

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to the human JARID1A (KDM5A) protein. Reacts with human and mouse by western blot and immunoprecipitation. It is predicted to react based on 100% sequence homology with rat and bovine.

Western blot

HeLa/iCas9-c1 control and KDM5A-KO cells.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.