This is a knockout-validated antibody summary, based on the publication "Maternal LSD1/KDM1A is an essential regulator of chromatin and transcription landscapes during zygotic genome activation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Immunofluorescence using anti-KDM1A antibody (red) at the zygote and two-cell stage shows nuclear accumulation of KDM1A in control embryos (top). Cre-mediated deletion of Kdm1a in maternal germline (bottom) leads to depletion of the protein after fertilization. Paternal pronucleus (p), maternal pronucleus (m) and polar body (pb) are indicated. DNA is counterstained by DAPI (blue). From [1].

Rabbit polyclonal IgG

Company: Abcam

Antibody: KDM1A

Catalog number: ab17721

Summary: Rabbit polyclonal IgG against a synthetic peptide derived from within residues 800 to the C-terminus of human LSD1.

Reacts with mouse, human, and pig. Suitable for western blot, immunoprecipitation, immunocytochemistry, immunohistochemistry (paraffin and frozen) and ChIP.

Immunocytochemistry

WT and Cre-mediated Kdm1a-deleted mouse embryos. Embryos were fixed in 4% paraformaldehyde, 0,2% sucrose, 0.04% Triton-X100 and 0.3% Tween20 in PBS for 15 min at 37°C.

1% BSA, 0.05% Triton-X100 and 30 min in 1% BSA in 0.05% Triton-X100; 1 mg/ml polyvinyl pyrrolidone (PVP; Sigma).

IF: 1:750 dilution in blocking buffer for ∼16 hr at 4°C.

1:500 dilution of Alexa568 goat anti-rabbit IgG/ Invitrogen A11036 for 2 hr at room temperature.

Inverted confocal microscope Zeiss (Germany) LSM700 with a Plan apo DICII (numerical aperture 1.4) 63x oil objective.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.