This is a knockout-validated antibody summary, based on the publication "Oncogenic Chromatin Modifier KAT2A Activates MCT1 to Drive the Glycolytic Process and Tumor Progression in Renal Cell Carcinoma", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

[enlarge]

Figure 1. The protein levels of KAT2A in A498 cells with KAT2A overexpression or deficiency were validated via Western blotting. From [1].

Rabbit monoclonal IgG

Company: Abcam

Antibody: KAT2A / GCN5

Catalog number: ab217876

Summary: Rabbit monoclonal antibody against a recombinant fragment of KAT2A. Reacts with mouse and human. It is predicted to work with rat. Suitable for western blot, immunoprecipitation and ChIP.

Western blot

WT and KAT2A KO A498 cells.

5% non-fat dry milk for 1 h.

1:2,000 dilution overnight.

1:5,000 dilution peroxidase-labeled anti-rabbit antibodies for 1 h.

ECL + plusTM Western Blotting system kit (cat. no. RPN2232; Amersham).