This is a knockout-validated antibody summary, based on the publication "Infertility-Causing Haploinsufficiency Reveals TRIM28/KAP1 Requirement in Spermatogonia", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Immunofluorescence staining of adult Trim28Ctr and Trim28iKO testes showing expression and absence of TRIM28 in wild-type and knockout SALL4-positive spermatogonia, respectively. Seminiferous tubules are demarcated by dotted lines. Scale bars, 20 mm. From [1].

Company: Abcam

Antibody:

Catalog number: ab22553

Summary:

Western blot

10% BSA, 3% milk, 0.1% Triton X-100 in PBS for 1 h at room temperature.

at 37°C for 1 h.

for 1 h at RT

The same clone (20C1) is sold as Abcam ab22553; Invitrogen MA1-2023.