This is a knockout-validated antibody summary, based on the publication "Epiplakin deficiency aggravates murine caerulein-induced acute pancreatitis and favors the formation of acinar keratin granules", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. (A–D) Immunofluorescence microscopy for epiplakin and K8 in pancreata of wild-type mice. Nuclear staining is shown for easier distinction of ductal and acinar cells. (M–P) Immunofluorescence microscopy for epiplakin and K8 in pancreata of K8-deficient (K8−/−) mice. Whole acini are outlined by dashed lines for orientation. Asterisks indicate cytosolic/perinuclear areas. Scale bars, 20 µm. From [1].

Rat monoclonal IgG2a

Company: Developmental Studies Hybridoma Bank

Antibody: K8

Catalog number: Troma I

Summary: Rat monoclonal IgG2a, kappa light chain, against mouse keratin type II (cytokeratin 8/18). Reacts with mouse and human protein. Suitable for western blot, immunoprecipitation, flow cytometry, immunofluorescence, and immunohistochemistry.

Immunohistochemistry

Pancreata of wild-type and K8-deficient (K8−/−) mice. Pancreata were fixed in 4% buffered formaldehyde (Agar, Stansted, UK) for 24 h, embedded, cut into 4 µm thick sections and subjected to antigen retrieval by boiling in EDTA buffer.

2% BSA.

1∶50 dilution for 1 h at room temperature.

Fluorophore-conjugated antibodies.

confocal laser-scanning microscope (Zeiss LSM 710) equipped with Plan-Apochromat 63x and 40x/1.4 NA oil-immersion objective lenses, using the ZEN software.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.