This is a knockout-validated antibody summary, based on the publication "Deletion of K1/K10 does not impair epidermal stratification but affects desmosomal structure and nuclear integrity", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal IgG1

Company: Progen

Antibody: K10

Catalog number: 11414

Summary: Mouse monoclonal IgG1 against cytokeratin 10. Reacts with human and mammals. Suitable for western blot, immunocytochemistry, immunohistochemistry (frozen and paraffin).

Western blot | Immunohistochemistry

Skin from wild-type and Krt1−/−/Krt10−/− mice. WB: Total protein was extracted from E18.5 skin in sample buffer (125 mM Tris-HCl, pH 6.8, 10% glycerol, 1% SDS). IHC: Cryosections (5 μm) of neonatal and E18.5 skin were fixed for 10 minutes with pre-cooled acetone at −20°C.

WB/IHC: 1:10 dilution.

WB: Horseradish-peroxidase-conjugated species-specific secondary antibodies (Dianova).

IHC: Species-specific Cy3-, Cy2-, Alexa-Fluor-594- or Alexa-Fluor-488-conjugated goat antibodies (Molecular Probes, Eugene, OR).

WB: Pierce Super Signal West Pico and Dura substrates (Thermo Scientific).

IHC: Axio Imager Z2 operated through Axiovision software (Zeiss, Hertfordshire, UK).

Please see Figure 1 in the article [1].

The same clone (DE-K10) is sold as BioLegend 904301; Santa Cruz Biotechnology sc-52318; Cell Sciences MON3025; LifeSpan Biosciences LS-C84110, LS-C95322, LS-C88282, LS-C88283, LS-C122024, LS-B7167, LS-B8116, LS-B8624; Abnova MAB12670, MAB12724, MAB12746; Abcam ab9026; Invitrogen MA5-13702, MA5-13705; BioLogo CK110; MyBioSource MBS531604; Dako M7002; Progen 11414.