JunD antibody | knockout validation | Abcam ab134067
DOI
//dx.doi.org/10.13070/ko.en.7.1989
Date
2017-01-20

This is a knockout-validated antibody summary, based on the publication "Tead and AP1 Coordinate Transcription and Motility", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

JunD antibody | knockout validation | Abcam ab134067 figure 1
Figure 1. Immunoblot analysis of cJun expression in HCT116 cells with and without expression of Cripsr knockout constructs against JunD. GAPDH was used as loading control. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Abcam

Antibody: JunD

Catalog number: ab134067

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to human JunD aa 100-200. Reacts with human. Suitable for western blot, immunoprecipitation, immunocytochemistry/immunofluorescence. Not suitable for immunohistochemistry (paraffin).

Validation Method

Western blot

Sample

Control and JunD-KO HCT116 cells.

Secondary incubation

HRP conjugated secondary antibodies (Jackson Laboratories).

Clone note

The same clone (EPR6520) is sold as LifeSpan Biosciences LS-B9020; Abcam ab134067; EMD Millipore MABE558.

References
  1. Liu X, Li H, Rajurkar M, Li Q, Cotton J, Ou J, et al. Tead and AP1 Coordinate Transcription and Motility. Cell Rep. 2016;14:1169-80 pubmed publisher