This is a knockout-validated antibody summary, based on the publications "JunB is essential for IL-23-dependent pathogenicity of Th17 cells", as cited below [1], "Conserved N-terminal cysteine dioxygenases transduce responses to hypoxia in animals and plants" for western blot knockout validation (figure 5b) [2] and "JUNB suppresses distant metastasis by influencing the initial metastatic stage" for immunohistochemistry knockout validation (figure 1c) [3]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Naive CD4+T cells from wild-type C57BL/6, Cd4CreJunBfl/fl and control JunBfl/fl mice were activated under TH17(β) conditions for 3 days, and JunB was detected by immunoblot analysis. From [1].

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: JunB

Catalog number: 3753

Summary: Rabbit monoclonal IgG, against a synthetic peptide corresponding to residues surrounding Pro169 of human JunB. Reacts with human, mouse, rat, and monkey. Suitable for western blot, immunoprecipitation, immunohistochemistry (paraffin), immunofluorescence/immunocytochemistry and chromatin IP.

Western blot

Cells were lysed with RIPA buffer (Thermo) containing complete protease inhibitor cocktail (4693159, Roche).

5% skim milk (Wako) in phosphate-buffered saline with 0.1% Tween-20 (Sigma).

1:2,000 dilution.

1:4,000 dilution horseradish peroxidase-conjugated anti-rabbit IgG antibodies (Cell Signaling Technology).

Clarity Western ECL (Bio-Rad) or SuperSignal West Femto detection reagents (Thermo) and an Las-3000 imaging system (Fuji film).