This is a knockout-validated antibody summary, based on the publication "Multiple Histone Lysine Methyltransferases Are Required for the Establishment and Maintenance of HIV-1 Latency", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot of JARID2 and β-actin (loading control) of mock and JARID2-KO E4 cells. From [1].

Rabbit monoclonal IgG

Company: Abcam

Antibody: Jarid2

Catalog number: ab192252

Summary: Rabbit monoclonal IgG against a synthetic peptide within human Jarid2 (aa 100-200). Reacts with human, and it is predicted to react with mouse and rat. Suitable for western blot and immunocytochemistry/immunofluorescence.

Western blot

Control and Jarid2-knockout E4 cells. Cells were lysed in radioimmunoprecipitation assay (RIPA) buffer (20 mM Tris (pH 7.5), 1% Triton X-100, 2 mM EDTA, 20 mM Tris-HCl (pH 8.1), 500 mM NaCl).

EZH2 (17-662; Millipore) and EHMT2 (3356S; Cell Signaling) are KO validated in the same Figure.