Jak2 antibody | knockout validation | Cell Signaling 3230

This is a knockout-validated antibody summary, based on the publication "JAK2 is dispensable for maintenance of JAK2 mutant B-cell acute lymphoblastic leukemias", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Jak2 antibody | knockout validation | Cell Signaling 3230 figure 1
Figure 1. Immunoblotting against the indicated target was performed against MHH–CALL4–Cas9 cells transduced with lentiviral vector (FgH1tUTG-GFP) expressing single guide RNAs (sgRNAs) targeting JAK2 (shJAK2.9901 or shJAK2.9903) at day 6 after sgRNA induction. β-Actin served as loading control. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: Jak2

Catalog number: 3230

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues surrounding Pro841 of Jak2. Reacts with human, mouse, and rat. It is predicted to react based on 100% sequence homology with hamster, monkey, chicken, Xenopus, bovine, dog, pig, and horse. Suitable for western blot, immunoprecipitation, immunohistochemistry (paraffin), immunofluorescence (immunocytochemistry) and flow cytometry.

Validation Method

Western blot

Sample

Control and Jak2 knockout MHH–CALL4 cells.

Detection

ECL reagents (Amersham ECL or ECL Prime, GE Healthcare) by film exposure (Fujifilm Super RX, Fujifilm) using an Agfa CP1000 developer (Agfa).

References
  1. Kim S, Knight D, Jones L, Vervoort S, Ng A, Seymour J, et al. JAK2 is dispensable for maintenance of JAK2 mutant B-cell acute lymphoblastic leukemias. Genes Dev. 2018;32:849-864 pubmed publisher