This is a knockout-validated antibody summary, based on the publication "HSF1 critically attunes proteotoxic stress sensing by mTORC1 to combat stress and promote growth", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit monoclonal IgG
Company: Cell Signaling
Antibody: JNK2
Catalog number: 9258
Summary: Rabbit monoclonal IgG against human JNK2/MBP fusion protein. Reacts with human, mouse, rat, hamster, monkey, and mink. Suitable for western blot.
Western blot
Control and Jnk2-/- primary MEFs. Whole-cell protein extracts were prepared in cold cell-lysis buffer (100 mM NaCl, 30 mM Tris-HCl pH 7.6, 1% Triton X-100, 20 mM sodium fluoride, 1 mM EDTA, 1 mM sodium orthovanadate, and Halt™ protease inhibitor cocktail from Thermo Scientific).
1:1,000 dilution in wash buffer overnight at 4°C.
1: 2,500 dilution peroxidase-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories) at room temperature for 1 hr
Signals were visualized by SuperSignal West chemiluminescent substrate (Thermo Scientific) followed by exposure to films.
Primary Jnk1-/- or Jnk2-/- MEFs were treated with 200 nM MG132 for 6 hr and analyzed with the indicated antibodies by western blot. JNK2 antibodies recognized both p54 and p46 isoforms. Please see Figure 2d in the article [1].
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