Itch antibody | knockout validation | BD Transduction Laboratories clone 32

DOI

//dx.doi.org/10.13070/ko.en.6.1796

Date

2016-11-08

This is a knockout-validated antibody summary, based on the publication "Itch inhibits IL-17-mediated colon inflammation and tumorigenesis by ROR-γt ubiquitination", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Mouse monoclonal

Company: BD Transduction Laboratories

Antibody: Itch

Catalog number: 611198 Clone 32/Itch

Summary: Mouse monoclonal antibody raised against a polypeptide corresponding to residues 114-220 of mouse Itch. Supplier recommended for WB and IF. Reacts with rat, human, mouse, and rabbit Itch.

Validation Method

Western blot

Sample

Lysates of colonic lamina propria lymphocytes (CLPLs)

Primary incubation

Anti-Itch antibody at 1:1000 dilution.

Detection

ECL reagent (Amersham) in conjunction with a BioRad ChemiDoc.

Figure

See figure 5 from the article [1].

Disclaimer

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.

References

Kathania M, Khare P, Zeng M, Cantarel B, Zhang H, Ueno H, et al. Itch inhibits IL-17-mediated colon inflammation and tumorigenesis by ROR-γt ubiquitination. Nat Immunol. 2016;: pubmed publisher

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