Itch antibody | knockout validation | BD 611198
DOI
//dx.doi.org/10.13070/ko.en.6.1829
Date
2016-11-20

This is a knockout-validated antibody summary, based on the publication "The E3 ligase Itch and deubiquitinase Cyld act together to regulate Tak1 and inflammation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Mouse monoclonal IgG1

Company: BD

Antibody: Itch

Catalog number: 611198

Summary: Mouse monoclonal IgG1 against mouse Itch (aa. 114-220). Reacts with rat, human, mouse, and rabbit. Recommended for western blot and immunofluorescence.

Validation Method

Western blot

Sample

WT, Itch−/− and Cyld−/− murine bone marrow derived macrophages (BMDMs). Cell lysis was performed using NP-40 buffer containing 50 mM Tris, pH8, 150 mM NaCl, 1% NP-40, EDTA-free protease inhibitor cocktail (Roche), 2 mM sodium fluoride (Boston Bioproducts), 10 mM-glycerophosphate (Boston Bioproducts), 1 mM sodium orthovanadate (Sigma).

Detection

Amersham ECL or ECL plus western blotting detection system (GE).

Figure

Please see Figure 5a in the article [1].

Disclaimer

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.

References
  1. Ahmed N, Zeng M, Sinha I, Polin L, Wei W, Rathinam C, et al. The E3 ligase Itch and deubiquitinase Cyld act together to regulate Tak1 and inflammation. Nat Immunol. 2011;12:1176-83 pubmed publisher