This is a knockout-validated antibody summary, based on the publication "Pioneering function of Isl1 in the epigenetic control of cardiomyocyte cell fate", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG2b
Company: DSHB
Antibody: Isl1
Catalog number: 39.4D5
Summary: Mouse monoclonal IgG2b against a recombinant fusion protein containing aa. 178-349 of rat Isl1 protein (C-terminus). It also detects Islet-2. Reacts with altricial fish larva, chicken, ferret, fish, frog, human, mouse, rat, and zebrafish. Suitable for western blot, immunofluorescence, immunohistochemistry (paraffin), chromatin immunoprecipitation, and gel supershift.
Western blot
Control and Isl1-/- cardiac progenitor cells (derived from mESCs). Cells were lysed with lysis buffer (50 mM Tris pH 7.5, 10 mM EGTA, 100 mM NaCl, 0.1% Triton X-100) plus protease and phosphatase inhibitors on ice.
1:10 dilution.
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