Invitrogen PA3-020 was examined in KO ES, mice and MEFs
DOI
http://dx.doi.org/10.13070/ko.en.6.1559
Date
2016-08-20

This is a knockout-validated antibody summary, based on the publication cited below. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD). If you are aware of any publication with knockout studies validating a monoclonal or recombinant antibody, either purchased from any supplier or developed by the author(s), please notify us through feedback.

Gene

FKBP52/FKBP52, heat shock protein 56 (HSP56).

Catalog number

PA3-020

Summary

PA3-020 is a rabbit polyclonal antibody that detects heat shock protein 56 (HSP56), also known as FKBP4/FKBP52, from human, monkey, mouse and rabbit tissues. The PA3-020 immunogen is a synthetic peptide corresponding to residues C(441) K D E R N D V A G S Q S Q V E T E A(458) of rabbit Heat Shock Protein 56. PA3-020 has been successfully used in Western blot and immunofluorescence procedures.

Company

Affinity Bioreagents, now part of Thermo Fisher.

Validation Method

Western blot.

Sample

FKBP52-KO ES (or mice) and MEFs.

Blocking agent

Information not available from the article.

Primary incubation

Information not available from the article.

Secondary incubation

Information not available from the article.

Detection

Information not available from the article.

Notes

A nonspecific band is observed under the one corresponding to FKBP52 in some westerns.

Figures

ES or mice (Fig 1), MEFs (Fig 5 and 9) from [1].

References
  1. Yang Z, Wolf I, Chen H, Periyasamy S, Chen Z, Yong W, et al. FK506-binding protein 52 is essential to uterine reproductive physiology controlled by the progesterone receptor A isoform. Mol Endocrinol. 2006;20:2682-94 pubmed