This is a knockout-validated antibody summary, based on the publication "CD81 Controls Beige Fat Progenitor Cell Growth and Energy Balance via FAK Signaling", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit monoclonal IgG
Company: Cell Signaling
Antibody: Integrin β5
Catalog number: 3629
Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues surrounding Lys789 of human integrin β5 protein. Reacts with human, mouse, rat, and monkey. Suitable for western blot, immunoprecipitation, and immunofluorescence/immunocytochemistry.
Western blot
Control and Itgb5 KO mice inguinal white adipose tissue.
5% milk or 3%–5% BSA in Tris-buffered saline with Tween 20 (TBS-T) for 1 hour at room temperature.
1:1,000 dilution overnight at 4C.
Anti-rabbit IgG (711-035-152, Jackson ImmunoResearch).
Immunoblotting for ITGB5 and b-actin in CD81+ cells from the inguinal white adipose tissue of Itgb5 KO or control mice. Please see Figure 4g in the article [1].
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