Integrin β1 antibody | knockout validation | Cell Signaling 34971

This is a knockout-validated antibody summary, based on the publication "CD81 Controls Beige Fat Progenitor Cell Growth and Energy Balance via FAK Signaling", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: Integrin β1

Catalog number: 34971

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues surrounding Pro680 of human integrin β1 protein. Reacts with human, mouse, and rat. Suitable for western blot and immunohistochemistry (paraffin).

Validation Method

Western blot

Sample

Control and Itgb1-KO inguinal white adipose tissue.

Blocking agent

5% milk or 3%–5% BSA in Tris-buffered saline with Tween 20 (TBS-T) for 1 hour at room temperature.

Primary incubation

1:1,000 dilution overnight at 4C.

Secondary incubation

Anti-rabbit IgG (711-035-152, Jackson ImmunoResearch).

Figure

Immunoblotting for ITGB1 and b-actin in control and Itgb1KO CD81+cells from the inguinal white adipose tissue of Itgb1flox/flox mice. Please see Figure 4e in the article [1].

References
  1. Oguri Y, Shinoda K, Kim H, Alba D, Bolus W, Wang Q, et al. CD81 Controls Beige Fat Progenitor Cell Growth and Energy Balance via FAK Signaling. Cell. 2020;: pubmed publisher