This is a knockout-validated antibody summary, based on the publication "Footprintless disruption of prosurvival genes in aneuploid cancer cells using CRISPR/Cas9 technology", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Rabbit monoclonal IgG

Company: CalBioreagents

Antibody: Id1

Catalog number: M085

Summary: Rabbit monoclonal IgG against recombinant full length mouse Id1 protein. Reacts with mouse and human. Suitable for western blot and immunohistochemistry.

Western blot

Control and Id1-KO Ret murine melanoma cell lines. Cells were lysed in 2% SDS, 125 mmol/L Tris–HCl (pH 6.8), and 10% glycerol, sonicated, then boiled for 5 min.

4% nonfat milk.

Horseradish-peroxidase-conjugated secondary antibodies (Dako, Hamburg, Germany),

Enhanced chemiluminescence (ECL) or AceGlow reagents (Thermo Fisher Scientific).

Please see Figure 4d in the article [1].