Id1 antibody Biocheck BCH-1/37-2
DOI
//dx.doi.org/10.13070/ko.en.6.1604
Date
2016-09-09

This is a knockout-validated antibody summary, based on the publications "Ablation of the transcriptional regulator Id1 enhances energy expenditure, increases insulin sensitivity, and protects against age and diet induced insulin resistance, and hepatosteatosis" [2] and "Id proteins regulate capillary repair and perivascular cell proliferation following ischemia-reperfusion injury" [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Antibody: Id1

Catalog number: BCH-1/37-2

Summary: Rabbit monoclonal against recombinant full length mouse Id1 protein. It does not react with human Id1. Other species have not been tested. Successfully used in western blot and immunohistochemistry.

Company: Biocheck, now part of OriGene

Article "Ablation of the transcriptional regulator Id1 enhances energy expenditure, increases insulin sensitivity, and protects against age and diet induced insulin resistance, and hepatosteatosis"
Validation Method

Western blot

Sample

different tissues of adult mice by using RIPA lysis buffer (89900; Thermo Fisher Scientific, Waltham, MA, USA), supplemented with complete mini-protease inhibitor tablet (11836153001; Roche, Mannheim, Germany).

Id1 antibody Biocheck BCH-1/37-2 figure 1
Figure 1. Western blot of Id1 expression in control kidneys from mice with indicated genotype: Id1−/−, Id3RFP/+ = Id1/Id3 knockout (KO), wild-type littermate = WT, TRE Id1 = TRE Id1, Tie2 rtTA mice. GAPDH is used as loading control, from [1].
Primary incubation

1:2500 dilution

Secondary incubation

anti-rabbit (W401B; Promega, Madison, WI, USA)

Figure

see Fig 1 in [2].

Article "Id proteins regulate capillary repair and perivascular cell proliferation following ischemia-reperfusion injury"
Validation Method

Western blot

Sample

kidneys from mice with indicated genotype: Id1−/−, Id3RFP/+ = Id1/Id3 knockout (KO)

Secondary incubation

Sheep anti-rabbit secondary antibodies (GE Healthcare, Piscataway, NJ)

References
  1. Lee D, Shenoy S, Nigatu Y, Plotkin M. Id proteins regulate capillary repair and perivascular cell proliferation following ischemia-reperfusion injury. PLoS ONE. 2014;9:e88417 pubmed publisher
  2. Satyanarayana A, Klarmann K, Gavrilova O, Keller J. Ablation of the transcriptional regulator Id1 enhances energy expenditure, increases insulin sensitivity, and protects against age and diet induced insulin resistance, and hepatosteatosis. FASEB J. 2012;26:309-23 pubmed publisher