This is a knockout-validated antibody summary, based on the publication "scRNA sequencing uncovers a TCF4-dependent transcription factor network regulating commissure development in mouse", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blot analysis of neocortical extracts from E18.5 WT or Tcf4KO mice using anti-TCF4 antibody. The blot presented is cropped. The longest isoform of TCF4 is missing in the KO samples (n=3). From [1].

Mouse monoclonal IgG2a κ

Company: Santa Cruz

Antibody: ITF-2

Catalog number: sc-393407

Summary: Mouse monoclonal antibody specific for an epitope mapping between amino acids 251-278 within an internal region of human ITF-2 (TCF4). Recommended for detection of ITF-2 isoforms SEF2-1A and SEF2-1D of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence and ELISA.

Western blot

WT and Tcf4KO mice neocortical extracts.

PBS with 0.1% Tween 20 (PBS-T).

1:500 dilution.

1:10,000 dilution anti-mouse HRP (Jackson ImmunoResearch, 115-035-003) PBST and incubated with the membranes for 1 h at RT.

Clarity Western Enhanced Chemiluminescence Substrate (Bio-Rad) and visualized with Fusion-SL (PeqLab).