ISGF3γ antibody | knockout validation

ISGF3γ antibody | knockout validation | BD Biosciences 610285

This is a knockout-validated antibody summary, based on the publication "IRAV (FLJ11286), an Interferon-Stimulated Gene with Antiviral Activity against Dengue Virus, Interacts with MOV10", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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ISGF3γ antibody | knockout validation | BD Biosciences 610285 figure 1

Figure 1. Western blot analysis of IRF9 expression in control A549 cells or IRF9 KO cells after DENV infection (72 h). Uninfected (-) and DENV-infected (+). GAPDH was used as a loading control. From [1].

Antibody information

Mouse monoclonal IgG1

Company: BD Biosciences

Antibody: ISGF3γ

Catalog number: 610285

Summary: Mouse monoclonal IgG1 against human ISGF3γ (p48) aa.126-351. Reacts with human by western blot and

immunofluorescence. Immunohistochemistry and immunoprecipitation are not recommended.

Validation Method

Western blot

Sample

Control and IRF9 KO A549 cells.

Blocking agent

Milk for 1 h.

Primary incubation

Overnight.

Secondary incubation

Horseradish peroxidase (HRP)-labeled secondary antibodies (Santa Cruz Biotechnology, Inc.).

Detection

SuperSignal West Femto maximum-sensitivity substrate (Pierce Biotechnology, Rockford, IL) on a LAS-3000 imaging system (Fujifilm, Edison, NJ).

References

Balinsky C, Schmeisser H, Wells A, Ganesan S, Jin T, Singh K, et al. IRAV (FLJ11286), an Interferon Stimulated Gene with Antiviral Activity Against Dengue Virus, Interacts with MOV10. J Virol. 2016;: pubmed

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