This is a knockout-validated antibody summary, based on the publication "Differential hepatic distribution of insulin receptor substrates causes selective insulin resistance in diabetes and obesity", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Protein levels of Irs1 and Irs2 in the hepatic PP and PV zones of LIrs2-KO mice in the fasting state. From [1].

Mouse monoclonal IgG1κ

Company: Millipore

Antibody: IRS-2

Catalog number: MABS15

Summary: Mouse monoclonal IgG1κ against a synthetic peptide corresponding to mouse IRS-2. Reacts with mouse and rat by western blot.

Western blot

Control and LIrs2-KO mouse liver. Frozen tissue was homogenized in buffer A (25 mM Tris-HCl, pH 7.4, 10 mM sodium orthovanadate, 10 mM sodium pyrophosphate, 100 mM sodium fluoride, 10 mM EDTA, 10 mM EGTA and 1 mM phenylmethylsulfonyl fluoride).

1:2,000 dilution.

Horseradish peroxidase-conjugated secondary antibodies.

ECL detection reagents (Amersham Biosciences, Buckinghamshire, UK).