IRG1 antibody | knockout validation | Abcam ab222411

This is a knockout-validated antibody summary, based on the publication "Immunoresponsive gene 1 modulates the severity of brain injury in cerebral ischaemia", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

IRG1 antibody | knockout validation | Abcam ab222411 figure 1
Figure 1. Primary MG generated from WT and IRG1−/− mice were stimulated with LPS 100 ng/ml for a time course followed by western blot analysis for IRG1 expression. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Abcam

Antibody: IRG1

Catalog number: ab222411

Summary: Rabbit monoclonal antibody against a recombinant fragment of IRG1. Reacts with mouse and human. Suitable for western blot and immunoprecipitation.

Validation Method

Western blot

Sample

WT and IRG1−/− mouse primary microglia (MG).

Detection

Immobilon Western Chemiluminescent HRP Substrate (Millipore, Temecula, CA, USA).

References
  1. Kuo P, Weng W, Scofield B, Furnas D, Paraiso H, Yu I, et al. Immunoresponsive gene 1 modulates the severity of brain injury in cerebral ischaemia. Brain Commun. 2021;3:fcab187 pubmed publisher