This is a knockout-validated antibody summary, based on the publication "Specific detection of interferon regulatory factor 5 (IRF5): A case of antibody inequality", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Immunoblot analysis of endogenous IRF5 protein expression in primary splenocytes from Irf5+/+ (WT) and Irf5−/− (KO) mice. β-actin levels and apparent molecular weight standards are shown as loading controls and for size comparison, respectively. Arrows indicate detection of an appropriately sized band(s) corresponding to murine IRF5. From [1].

Rabbit polyclonal

Company: Cell Signaling

Antibody: IRF5

Catalog number: 4950

Summary: Rabbit polyclonal against a synthetic peptide corresponding to residues surrounding Ser172 of mouse IRF-5 protein. Reacts with mouse and rat protein. Suitable for western blotting and immunoprecipitation.

Western blot

Primary splenocytes from Irf5+/+ (WT) and Irf5−/− (KO) mice.

5% BSA in TBS/0.05% Tween 20 for 1 h.

Overnight at 4 °C.

Horseradish peroxidase conjugated secondary antibodies (Cell Signaling: α-rabbit #7074S).

Clarity™ ECL Western Blotting Substrate (Bio-Rad Laboratories) and chemiluminescence detected with a ChemiDoc™ MP Imaging System (Bio-Rad Laboratories).

The antibody does not work in IHC. Other IRF5 antibodies PT10547-1-AP (Proteintech), IC8447P (R&D), and SC98651 (Santa Cruz) were unable to detect differences in IRF5 expression between WT and KO splenocytes.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.