This is a knockout-validated antibody summary, based on the publication "The NEMO adaptor bridges the nuclear factor-kappaB and interferon regulatory factor signaling pathway", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blot of MEF lysates from cells WT for Irf3 and those deleted for irf3 (Irf3 -/- and Irf3 -/-, Irf9 -/-). Probed with anti-Irf3 antibody. Courtesy of the authors.

Rabbit polyclonal

Company: Santa Cruz Biotechnology

Antibody: IRF3

Catalog number: SC-9082 / FL-425

Summary: Rabbit polyclonal antibody raised against resides 1-425 of human IRF3. Supplier recommended for WB, IP, IF and elisa. Reacts with mouse, rat and human IRF3.

Note: this antibody will be discontinued December 31, 2016

Western blot

Lysates of MEFs from Irf3 wild-type and Irf3 -/- and Irf3 -/-, Irf9 -/- animals.

PBS containing 5% milk powder and 0/1% Tween 20.

Anti-Irf3 antibody at 1μg/ml in PBS containing 5% milk powder.

HRP-conjugated anti-rabbit IgG (Amersham) at 1:3000 dilution in blocking buffer.

ECL reagent.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.