This is a knockout-validated antibody summary, based on the publication "Interferon-Independent Upregulation of Interferon-Stimulated Genes during Human Cytomegalovirus Infection is Dependent on IRF3 Expression", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. IRF3 KO clones generated by CRISPR/Cas-9 were screened by immunoblot for IRF3 alongside the parental Cas-9 hTERT HFs with GAPDH as a loading control. From [1].

Mouse monoclonal IgG1 κ

Company: Santa Cruz

Antibody: IRF-3

Catalog number: sc-376455

Summary: Mouse monoclonal IgG1 κ specific for an epitope mapping between amino acids 389-427 near the C-terminus of human IRF-3. Recommended for detection of IRF-3 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA.

Western blot

Control and IRF3 KO HFF-1 primary human foreskin fibroblasts (HFs).

1:1,000 dilution.

1:2,000 dilution horseradish peroxidase-lined Donkey anti-rabbit IgG (406401, Biolegend, San Diego, CA, USA).

Clarity™ Western ECL Substrate (Bio-Rad, Hercules, CA, USA) before imaging with a Bio-Rad ChemiDoc™ MP.