This is a knockout-validated antibody summary, based on publications "The convergent roles of NF-κB and ER stress in sunitinib-mediated expression of pro-tumorigenic cytokines and refractory phenotype in renal cell carcinoma", as cited below [1], "Pharmacological induction of selective endoplasmic reticulum retention as a strategy for cancer therapy" for western blot knockout validation (figure 1f, s4a) [2], "Repression of viral gene expression and replication by the unfolded protein response effector XBP1u" for western blot knockout validation (see figure 5a [3] ), "Transforming growth factor β (TGFβ) cross-talk with the unfolded protein response is critical for hepatic stellate cell activation" for western blot knockout validation (figure 1c,2a) [4], and "Carfilzomib modulates tumor microenvironment to potentiate immune checkpoint therapy for cancer" for western blot knockout validation (figure e3c) [5]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot analysis of cytoplasmic extracts from wild type (WT) 786-O cells and their knockout counterparts treated with 10 μM of sunitinib (Sun). From [1].

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: IRE1α

Catalog number: 3294

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues surrounding His963 of human IRE1α. Reacts with human and mouse by western blot and immunoprecipitation.

Western blot

Wild type and IRE1a-KO 786-O cells.

5% nonfat dry milk overnight.

For 2 h.

Horseradish peroxidase-conjugated secondary antibody for 30 min.

Enhanced chemiluminescence (ECL Western Blotting Kit, Amersham).