IRAK4 antibody | knockout validation | Pierce (Thermo Fisher) MA5-15883
DOI
//dx.doi.org/10.13070/ko.en.6.1871
Date
2016-11-25

This is a knockout-validated antibody summary, based on the publication "Comprehensive RNAi-based screening of human and mouse TLR pathways identifies species-specific preferences in signaling protein use", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Mouse monoclonal

Company: Pierce (Thermo Fisher Scientific)

Antibody: IRAK4

Catalog number: MA5-15883 (Clone 2H9)

Summary: Mouse monoclonal antibody raised against an E.coli-expressed recombinant fragment of human IRAK4. Supplier recommended for flow cytometry, IHC and WB. Reacts with human, mouse and primate IRAK4.

Validation Method

Western blot

Sample

Lysates of wild-type and IRAK4 -/- THP-1 cells.

Primary incubation

Anti-IRAK4 antibody.

Secondary incubation

HRP-conjugated secondary antibody.

Figure

See Figure S5 in [1].

Clone note

The same clone (2H9) is sold as LifeSpan Biosciences LS-B10452; Abnova MAB10701; Invitrogen MA5-15883.

References
  1. Sun J, Li N, Oh K, Dutta B, Vayttaden S, Lin B, et al. Comprehensive RNAi-based screening of human and mouse TLR pathways identifies species-specific preferences in signaling protein use. Sci Signal. 2016;9:ra3 pubmed publisher