This is a knockout-validated antibody summary, based on the publication "Comprehensive RNAi-based screening of human and mouse TLR pathways identifies species-specific preferences in signaling protein use", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit monoclonal
Company: Cell Signaling Technology
Antibody: IRAK1
Catalog number: 4504 (Clone D51G7)
Summary: Rabbit monoclonal antibody raised against a synthetic peptide corresponding the C-terminal region of mouse IRAK1.
Supplier recommended for WB, IP and flow cytometry.
Reacts with human, mouse and monkey IRAK1.
Western blot
Lysates of wild-type and IRAK1 -/- THP-1 cells.
Anti-IRAK1 antibody.
HRP-conjugated secondary antibody.
See Figure 7 in [1].
If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.
- If you are aware of any publication with knockout studies validating a monoclonal or recombinant antibody, either purchased from a supplier or developed by the author(s), please notify us through feedback.