IQGAP1 antibody | knockout validation | Abcam ab133490

This is a knockout-validated antibody summary, based on the publication "The scaffold protein IQGAP1 is crucial for extravasation and metastasis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

IQGAP1 antibody | knockout validation | Abcam ab133490 figure 1
Figure 1. Western blot of IQGAP1 and GAPDH in parental (WT) and clonal IQGAP1 knockout (sg) LM2 lines. Molecular weight markers in kDa are indicated. Full-length IQGAP1 is shown with an arrow. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Abcam

Antibody: IQGAP1

Catalog number: ab133490

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues in human IQGAP1. Reacts with human and mouse. Suitable for western blot, immunohistochemistry (paraffin), immunocytochemistry/immunofluorescence, and flow cytometry. Unsuitable for immunoprecipitation.

Validation Method

Western blot

Sample

WT and IQGAP1 KO LM2 cell lines. Cells were lysed in Cell Lysis Buffer (Cell Signaling Technology) containing a complete mini-protease inhibitor cocktail (Roche) and a phosphatase-inhibitor cocktail (PhosSTOP, Roche).

Primary incubation

1:1,000 dilution.

Detection

Tanon 5200CE Chemi-Image System (Tanon).

References
  1. Hebert J, Tian C, Lamar J, Rickelt S, Abbruzzese G, Liu X, et al. The scaffold protein IQGAP1 is crucial for extravasation and metastasis. Sci Rep. 2020;10:2439 pubmed publisher