This is a knockout-validated antibody summary, based on the publications "IP3 receptors regulate vascular smooth muscle contractility and hypertension" (discussed below) [1] and "IP3 Receptors Preferentially Associate with ER-Lysosome Contact Sites and Selectively Deliver Ca2+ to Lysosomes" (western blot, see supplementary figure 5a) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Immunoblot of IP3R3 in macrophages from control and IP3R1-3 TKO mice. Courtesy of the Authors [1].

Mouse monoclonal IgG2a

Company: BD Biosciences

Antibody: IP3R-3

Catalog number: 610312

Summary: Mouse monoclonal IgG2a against human IP3R-3 (aa. 22-230). Reacts with human, mouse, rat, dog, and cow. Suitable for western blot, immunofluorescence,and immunoprecipitation. Immunohistochemistry-formalin (antigen retrieval required) is not recommended.

Western blot

Protein lysates were prepared from smooth muscle layers of thoracic aortas or cultured VSMCs using a protein lysis buffer containing 8 M urea, 2 M thiourea, 3% SDS, 75 mM DTT, 0.05 M TrisHCl (pH 6.8), and 0.03% bromophenol blue.