This is a knockout-validated antibody summary, based on the publication " Disease severity in K/BxN serum transfer-induced arthritis is not affected by IL-33 deficiency", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. From Figure 2 in [1]. Knee joint from wild-type and Il-33 knockout mice were stained using anti-Il-33 antibody.

Goat polyclonal

Company: R&D Systems

Antibody: IL-33

Catalog number: AF3626

Summary: Goat polyclonal antibody raised against an E. coli-expressed recombinant polypeptide corresponding to residues 109-266 of mouse IL-33.

Supplier recommended for WB, IHC, elisa and flow cytometry.

Reacts with mouse IL-33.

Immunohistochemistry

Synovium of knee joints from wild-type and IL-33 knockout mice.

Anti-IL-33 antibody diluted to 1g/ml in Antibody Diluent (S2022, Dako).

HRP-conjugated anti-goat IgG (1:500)(sc-2304, Santa Cruz Biotechnology).

Diaminobenzidine.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.