This is a knockout-validated antibody summary, based on the publication "The Golgi apparatus acts as a platform for TBK1 activation after viral RNA sensing", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal
Company: Cayman Chemical
Antibody: IKKε/TBK1
Catalog number: 13929. This product is no longer available from Cayman Chemical. The same clone 72B587 is available from multiple suppliers. Labome is providing unique names for antibody clones. This clone is designated as IKBK07Img1Mo.
Summary: Mouse monoclonal IgG against a synthetic peptide corresponding to amino acids 175-188 of human IKKε/TBK1/NAK/IKBKE/IKKI/IKKE.
Immmunocytochemistry.
Cells grown on coverslips were fixed in 4 % paraformaldehyde in phosphate buffered saline (PBS) for 10 minutes, and then permeabilized by incubation with 0.15 % Triton X-100 in PBS for 15 minutes.
Non-specific binding sites were blocked by incubating cells in a solution of 2 % BSA in PBS for 1 hour.
1 hour at room temperature or overnight at 4 °C.
A Leica SP5 confocal microscope (Leica Microsystems, Wetzlar, Germany) equipped with a 63× oil immersion fluorescence objective.
The same clone (72B587) is sold as Santa Cruz Biotechnology sc-52931; Enzo Life Sciences ALX-804-321-C100; Rockland Immunochemicals 200-301-H85; Active Motif 40950; LifeSpan Biosciences LS-C368278.
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