This is a knockout-validated antibody summary, based on the publication "The Golgi apparatus acts as a platform for TBK1 activation after viral RNA sensing", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. TBK1+/+ and TBK1–/– mouse embryonic fibroblasts detected with phosphoTBK1 Abcam ab109272 antibody. From Figure 1f in [1]. According to the authors, Cell Signaling Technology 5483 displayed the same results.

Rabbit monoclonal IgG

Company: Abcam

Antibody: phosphorylated IKKε/TBK1

Catalog number: ab109272

Summary: The immunogen is a synthetic phosphopeptide corresponding to residues surrounding Ser172 of human TBK1. Species Reactivity: human, mouse, rat.

Immmunocytochemistry.

Cells grown on coverslips were fixed in 4 % paraformaldehyde in phosphate buffered saline (PBS) for 10 minutes, and then permeabilized by incubation with 0.15 % Triton X-100 in PBS for 15 minutes.

Non-specific binding sites were blocked by incubating cells in a solution of 2 % BSA in PBS for 1 hour.

1 hour at room temperature or overnight at 4 °C.

A Leica SP5 confocal microscope (Leica Microsystems, Wetzlar, Germany) equipped with a 63× oil immersion fluorescence objective.