This is a knockout-validated antibody summary, based on the publication "The NEMO adaptor bridges the nuclear factor-kappaB and interferon regulatory factor signaling pathways" [1] (discussed below), "MAVS activates TBK1 and IKKε through TRAFs in NEMO dependent and independent manner" [2] (see figure 7e in the article) and "Priming Phosphorylation of TANK-Binding Kinase 1 by IkB Kinase beta Is Essential in Toll-Like Receptor 3/4 Signaling" for western blot knockout validation (figure 4g) [3]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blot of MEF lysates from IKKbeta KO (Ikbkb -/-) cells infected with empty retroviral vector (+Vector) or retroviral vector encoding IKKbeta (+WT). IKKbeta is also detected in lysates of cells deleted for Ikbkg (NEMO), Irf3 or Irf3 and Irf9. Probed with anti-IKKbeta antibody (row 4). Courtesy of the authors.

Rabbit monoclonal

Company: Cell Signaling Technology

Antibody: IKKbeta

Catalog number: 2370, Clone 2C8

Summary: Rabbit monoclonal antibody raised against a synthetic peptide corresponding to the C-terminal region of human IKKbeta. Supplier recommended for WB. Reacts with human, mouse rat and monkey IKKbeta.

Western blot

Lysates of MEFs from wild-type and IKKbeta knock out (Ikbkb -/-) mice.

PBS containing 5% milk powder and 0/1% Tween 20.

Anti-IKKbeta antibody at 1μg/ml in PBS containing 5% milk powder.

HRP-conjugated anti-rabbit IgG (Amersham) at 1:3000 dilution in blocking buffer.

ECL reagent.