This is a knockout-validated antibody summary, based on the publication "Mitochondrial permeabilization engages NF-κB-dependent anti-tumour activity under caspase deficiency", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rat monoclonal IgG2A
Company: R&D systems
Antibody: IKK beta
Catalog number: MAB7155
Summary: Rat monoclonal antibody against a synthetic peptide corresponding to mouse IKK beta (Val530-Asp757). Reacts with mouse by western blot.
Western blot
Control and IKKβ deleted BCL-xL dependent SVEC cells. Cells were lysed in NP-40 lysis buffer (1% NP-40, 1mM EDTA, 150mM NaCl, 50 mM Tris pH 7.4, 1 mM PMSF and complete protease inhibitors (Roche)).
2% BSA/PBS for 1 hour at room temperature.
1/1000 dilution overnight in 4°C.
HRP-linked secondary antibodies.
Cell lysates from vector or IKKβ deleted BCL-xL dependent SVEC cells were immunoblotted for IKKβ and beta-Actin expression. Please see Suppl. Figure 2d in the article [1].
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- geneproduct